| 质粒名称: | pMSCV-IRES-mCherry FP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 52114 |
| 存储实验室: | Dario Vignali |
|
载体骨架基本信息 |
|
| 载体名称: | MSCV IRES GFP |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 6502bp |
| 载体类型: | 哺乳细胞表达, Retroviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 标签/融合蛋白: | IRES-mCherry (C terminal on backbone) |
| 克隆方法: | 限制性内切酶 |
| 5’测序引物 | pLXSN_5_primer |
|
文献引用方法 |
|
| 材料和方法部分: | pMSCV-IRES-mCherry FP was a gift from Dario Vignali (Addgene plasmid # 52114 ; http://n2t.net/addgene:52114 ; RRID:Addgene_52114) |
买家导航
pMSCV-IRES-mCherry FP载体质粒基本信息
pMSCV-IRES-mCherry FP质粒图谱载体图谱和pMSCV-IRES-mCherry FP载体序列质粒序列多克隆位点信息
pMSCV-IRES-mCherry FP质粒载体简介
pMSCV-IRES-mCherry FP质粒序列载体序列
LOCUS pMSCV-IRES-mCherry FP 6502 bp DNA circular SYN 14-APR-2025
DEFINITION a bicistronic IRES-mCherry containing retroviral vector.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6502)
TITLE Vignali lab plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6502)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT Sequence Label: pMSCV-IRES-mCherry FP
FEATURES Location/Qualifiers
source 1..6502
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 134..153
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 387..404
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 521..542
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter join(557..562,563..580,581..587)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 595..611
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
LTR 773..1287
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
CDS complement(1448..2158)
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(1726..1745)
/label=mCherry-F
/note="mCherry, forward primer"
primer_bind 1995..2013
/label=mCherry-R
/note="mCherry, reverse primer"
misc_feature 2161..2710
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(2320..2339)
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
primer_bind 2549..2566
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
CDS complement(2779..3195)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(2795..2811)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(2827..2849)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 3262..3603
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
LTR 3667..4183
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(4383..4402)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(4530..4552)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(4746..4765)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4865..4887
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4925..4943)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5011..5115
/gene="bla"
/label=AmpR promoter
CDS join(5116..5184,5185..5976)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5334..5353)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin join(6147..6502,1..233)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
61 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
601 gcggataaca atttcacaat tctccccagc tctttctgcg ctgtccataa tgaactattt
661 caggagtatt ggagtatgtg gattcaaaac agtggctaat tgagtcagga ttacttaata
721 ttcgactcct accctcagaa tgttgttctc caacttcaag aaactgttac ccatgaaaga
781 cccccgctga cgggtagtca atcactcaga ggagaccctc ccaaggaaca gcgagaccac
841 aagtcggatg caactgcaag agggtttatt ggatacacgg gtacccgggc gacgcagtct
901 atcggaggac tggcgcgccg agtgaggggt tgtgggctct tttattgagc tcggggagca
961 gaagcgcgcg aacagaagcg agaagcgaac tgattggtta gttcaaataa ggcacagggt
1021 catttcaggt ccttggggca ccctggaaac atctgatggt tctctagaaa ctgctgaggg
1081 cgggaccgca tctggggacc atctgttctt ggccctgagc cggggcagga actgcttacc
1141 acagatatcc tgtttggccc atattctgct gtctctctgt tcctaacctt gatctgaact
1201 tctctattct cagttatgta ttttccatgc cttgcaaaat ggcgttactt aagctagctt
1261 gccaaaccta caggtggggt ctttcattcc cccctttttc tggagactaa ataaaatctt
1321 ttattttatc gatatcagcg ctttaaattt gcgcatgcta gctatagttc tagaggtacc
1381 ggttgttaac gttagccggc tacctgaagc ttgcatgcct gcaggtcgac tctagagtcg
1441 cggccgctta cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt
1501 cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga
1561 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag
1621 cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc
1681 cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catcgtcttc ttctgcatta
1741 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
1801 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc
1861 gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt
1921 gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg
1981 gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
2041 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
2101 tgaagcgcat gaactccttg atgatggcca tgttatcctc ctcgcccttg ctcaccatgg
2161 tattatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc tagacgtttt
2221 tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc agatcccata
2281 caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga ggagagccat
2341 ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc cgcctttgca
2401 ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg tggggggttc
2461 cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc cagaggaact
2521 gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga aagaccccta
2581 ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc aaaagacggc
2641 aatatggtgg aaaataacat atagacaaac gcacaccggc cttattccaa gcggcttcgg
2701 ccagtaacgt tagggggggg gcggaattga tcccgctcga gcaattggga tccgctagct
2761 acgtaagatc tgaattccgg cgcctagaga aggagtgagg gctggataaa gggaggatcg
2821 aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag gaagaggagg
2881 cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag ccaaggcttc
2941 ccaggtcacg atgtagggga cctggtctgg gtgtccatgc gggccaggtg aaaagacctt
3001 gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc atccgacgtt
3061 aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga catctaccga
3121 ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac ttaagggagt
3181 ggtaacagtc tggccctaat tttcagacaa atacagaaac acagtcagac agagacaaca
3241 cagaacgatg ctgcagcgct gcagcagaca agacgcgcgg cttcggttcc aaaccgaaag
3301 caaaaattca gacggaggcg ggaactgttt taggttctcg tctcctacca gaaccacata
3361 tcctgacggg gtcggattcc acatcgactc ccttcctcag gtcgggccac aaaaacggcc
3421 cccaaagtcc ctgggacgtc tcccagggtt gcggccgggt gttcagaact cgtcagttcc
3481 accacgggtc cgccagatac agagctagtt agctaactag tacagacgca ggcgcaaaca
3541 ttagatgccg gcacaaacac gcacaaagac agagacagac acgaaacgac cgctggccag
3601 cttacctccc ggcggggggg tcggtggtcc ctgggcaggg gtctccaaat ctcggtggaa
3661 cctccaaatg aaagaccccc gaggtgggca gtcaatcaat ctgaggagac cctcccaagg
3721 atcagcgagt ccacgattcg gatgcaaaca gcaagaggct ttattgggaa tacgggtacc
3781 cgggcgacgc agtctatcgg aggactggcg cgccgagtga ggggttgtgg gctcttttat
3841 tgagctcggg gagcagaagc gcgcgaacag aagcgagaag cgaactgatt ggttagttca
3901 aataaggcac agggtcattt caggtccttg gggcaccctg gaaacatctg atggttctct
3961 agaaactgct gagggcggga ccgcatctgg ggaccatctg ttcttggccc tgagccgggg
4021 caggaactgc ttaccacaga tatcctgttt ggcccatatt ctgctgtctc tctgttccta
4081 accttgatct gaacttctct attctcagtt atgtattttc catgccttgc aaaatggcgt
4141 tacttaagct agcttgccaa acctacaggt ggggtctttc attcccccct ttttctggag
4201 actaaataaa atcttttatt ttatctatgg ctcgtactct ataggcttca gctggtgata
4261 ttgttgagtc aaaactagag cctggaccac tgatatcctg tctttaacaa attggactaa
4321 tcgcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
4381 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
4441 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
4501 ctccttgcat gcaccattcc ttgcgccgtc gttttacaac gtcgtgactg ggaaaaccct
4561 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
4621 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
4681 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
4741 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4801 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4861 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4921 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4981 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
5041 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
5101 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5161 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5221 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5281 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5341 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5401 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5461 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5521 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5581 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5641 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5701 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5761 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5821 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5881 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5941 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
6001 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
6061 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6121 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6181 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6241 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6301 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6361 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6421 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6481 acacagccca gcttggagcg aa
//
